Assessing large-vessel endothelial permeability using near-infrared fluorescence imaging--brief report.

Atheroma Susceptible to Thrombosis Exhibit Impaired Endothelial Permeability In Vivo as Assessed by Nanoparticle-Based Fluorescence Molecular Imaging.

BACKGROUND The role of local alterations in endothelial functional integrity in atherosclerosis remains incompletely understood. This study used nanoparticle-enhanced optical molecular imaging to probe in vivo mechanisms involving impaired endothelial barrier function in experimental atherothrombosis. METHODS AND RESULTS Atherosclerosis was induced in rabbits (n=31) using aortic balloon injur...

Evaluation of Reflection Optical Imaging Characteristics Using Fluorescence with Near Infrared Wavelength at Different Depths of Tissue Equivalent Phantom

Temperature mapping near plasmonic nanostructures using fluorescence polarization anisotropy.

We report on a thermal imaging technique based on fluorescence polarization anisotropy measurements, which enables mapping the local temperature near nanometer-sized heat sources with 300 nm spatial resolution and a typical accuracy of 0.1 degrees C. The principle is demonstrated by mapping the temperature landscape around plasmonic nano-structures heated by near-infrared light. By assessing di...

Multimodality tumor imaging targeting integrin αvβ3

The cell adhesion molecule integrin αvβ3 is an important player in the process of tumor angiogenesis and metastasis. Antibodies, peptides, peptidomimetics, and small molecule antagonists against integrin αvβ3 have been shown to induce endothelial apoptosis, to inhibit tumor angiogenesis, and to increase endothelial permeability. The ability to quantitatively image integrin αvβ3 expression in vi...

Non-invasive Imaging of Endothelial Progenitor Cells in Tumor Neovascularization Using a Novel Dual-modality Paramagnetic/Near-Infrared Fluorescence Probe

OBJECTIVE Bone-marrow derived endothelial progenitor cells (EPCs) play an important role in tumor neovasculature. Due to their tumor homing property, EPCs are regarded as promising targeted vectors for delivering therapeutic agents in cancer treatment. Consequently, non-invasive confirmation of targeted delivery via imaging is urgently needed. This study shows the development and application of...